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GM Buffer 蛋白質相關緩衝溶液

GM Buffer

蛋白質相關緩衝溶液

Protein Related Buffers & Reagents

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商 品 說 明 資 料

 

Product Name

 Description

Composition

Order Information

10X TG Buffer

(Tris-Glycine/Transfer Buffer)

TG buffer is the electophoresisbuffer for polyacrylamidegels, that is not including SDS for denatured protein in the PAGE. TG buffer is prepared as a 10X concentrate and stored at room temperature.

  

0.25 M Tris, 1.92 M Glycine, pH 8.3

 

GB04-1, 1L

10X TGS Buffer

(Tris-Glycine-SDS Buffer)

TGS buffer is the electophoresisbuffer for polyacrylamidegels, that is including SDS for denatured protein in the PAGE. TGS buffer is prepared as a 10X concentrate and stored at room temperature.

  

0.25 M Tris, 1.92 M Glycine, 1% SDS, pH 8.3

 

GB05-1, 1L

10X PBS Buffer

(Phosphate Buffered Saline)

PBS buffer is prepared as a 10X concentrate and stored at room temperature.

 

1.37 M Sodium Chloride, 27 mM Potassium Chloride, 43 mM Na2HPO4 ・7H2O, 14 mM KH2PO4, pH 7.4±0.05

GB07-1, 1 L

GB07-4, 4 L

10X TBS Buffer

(Tris Buffered Saline)

TBS buffer is prepared as a 10X concentrate and stored at room temperature and that applicatedin detection of protein by Western blot.

  

30 g/L Tris, 80 g/L Sodium chloride, 2 g/L Potassium chloride

GB08-1, 1 L

10X TBST Buffer (Tris Buffered with Tween 20)

TBST buffer is prepared as a 10X concentrate and stored at room temperature, and that applicatedin detection of protein by Western Blot.

  

30 g/L Tris,

80 g/L Sodium chloride,

2 g/L Potassium chloride,
10 ml/L Tween 20

GB08T-1, 1 L

Instant Blue Gel Staining Reagent

 

InstantBlue™ Gel Staining Reagent is a convenient alternative to traditional Coomassie Blue staining procedures, based on a colloidal G250 formulation.

 

For More Information, please refer to: Instant Blue

GM49, 500 ml

5X Protein Loading Buffer

GeneMark 5X ProteinSample Buffer is especially formulated for use in protein sample preparations for  SDS-PAGE system. A protein sample is mixed with the 5X sample buffer (4:1) and is boiled (or heated) on a heating block for 2~5 min. 

  

10% SDS, 50% glycerol, 0.05 M DTT, 0.01 M EDTA, 0.05% bromophenol blue and 0.125 M Tris HCl, pH approx. 6.8

GM47-b-50, 50 ml

GM47-b-100, 100 ml

TMB Solution

TMB is the most popular chromogenic substrate for horseradish peroxidase (HRP). TMB is used in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).

  

50 ml of TMB Substrate

(1 mg/ml) in DMSO

450 ml of Substrate Dilution Buffer

GM61

BSA Standard Solution

  

1 mg / ml

GD0069-2

GD0069-10

4X Resolving Buffer

4X Resolving Buffer is used for the preparation of gradient, continuous and discontinuous denaturating polyacrylamide gels. Store at 4°C.

  

1.5 M Tris-HCl pH 8.8, 10%SDS

GPB06, 500 ml

4X Stacking Buffer

4X Stacking Buffer is used for the preparation of gradient, continuous and discontinuous denaturating polyacrylamide gels. Store at 4°C.

  

0.5 MTris-HCl pH 6.8, 10%SDS

GPB07, 500 ml

Blocking Buffer

GeneMark Blocking Buffer can minimize the non-specific signal in Western blot assay, for blocking the remaining surface of nylon or PVDF membrane. It can be used as a diluent for primary and secondary antibodies. 

Store at 4°C

  

10 mM Tris-HCl, 0.9% NaCl, 0.1% Triton X-100, 0.25% gelatin, 0.02% SDS

GPB04, 500 ml

10X Transfer Buffer

1X Formulation: 25 mM Tris, 192 mM Glycine, 20% (v/v) methanol

GPB05, 500 ml

Stripping Buffer (Mild)

Stripping Buffer is used in removal of primary and secondary antibodies from a Western blot membrane. The PVDF membrane is highly recommended, because it can minimize loss of sample protein. Use chemiluminescent reagents such as ECL are recommended as these reagents will not leave a stain on the membrane, and are more sensitive than colorimetric reagents. Store at 4°C.

  

1.5% Glycine, 0.1% SDS, and 1% Tween-20, pH 2.2

GPB08, 500 ml

Stripping Buffer (Harsh)

Stripping Buffer is used in removal of primary and secondary antibodies from a Western blot membrane. The PVDF membrane is highly recommended, because it can minimize loss of sample protein. Use chemiluminescent reagents such as ECL are recommended as these reagents will not leave a stain on the membrane, and are more sensitive than colorimetric reagents. Store at 4°C.

  

0.063 M Tris, 2% SDS, and 0.8% ß-me, pH 2.2

GPB09, 100 ml

0.5 M Tris pH 6.8

0.5 M Tris Buffer (pH 6.8), autocleaved, is used in PAGE gel preparation. Store at room temperature

 

0.5 M Tris, pH 6.8

GPB03, 500 ml

1 M Tris pH 6.8

1 M Tris Buffer (pH 6.8), autocleaved, is used in PAGE gel preparation. Store at room temperature.

 

1 M Tris, pH 6.8

GPB02, 500 ml

1 M Tris pH 7.2

1 M Tris, pH 7.2

GB39, 1 L

1 M Tris pH 7.4

1 M Tris, pH 7.4

GB40, 1 L

1 M Tris pH 7.5

1 M Tris, pH 7.5

GB41, 1 L

1 M Tris pH 8.0

1 M Tris, pH 8.0

GB42, 1 L

1.5 M Tris pH 8.8

1.5 M Tris Buffer (pH 8.8), autocleaved, is used in PAGE gel preparation. Store at room temperature.

1.5 M Tris, pH 8.8

GPB01, 500 ml

2.0 M Tris pH 8.0

2.0 M Tris, pH 8.0

GPB10, 500 ml


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