High signal sensitivity and long signal duration for chemiluminescent

LumiFlash™ Ultima Chemiluminescent Substrate, HRP is an advanced ECL product in terms of efficacy and sensitivity for chemiluminescent detection of immobilized proteins (Western blotting and ELISA). LumiFlash™ Ultima Chemiluminescent Substrate, HRP provides high sensitivity and long signal duration in Western blotting application. It needs very short exposure time to the X-ray film or other documentation systems, and can get low background and high signal with clear image. LumiFlash™ Ultima Chemiluminescent Substrate, HRP is the perfect choice for most Western blotting application.

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Special Characteristic

• High signal sensitivity –  detection of target protein at low-picogram level on WB and ELISA application

• Antibody saving – diluted lower antibody concentration for WB and ELISA application

• Long duration – provide stable signal for long exposure

• Economy – cost less than other ECL substrate with similar sensitivity level 

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Product Detail :

Figure 1. The comparison of WB application for LumiFlash Ultima, VisGlow Plus and Luminata Crescendo.

K562 cell lysate with 1/2 serial dilution from 50 µg was separated by 12.5% SDS-PAGE and probed by anti HSP 47. All results were exposed to X-ray film for 3 minutes.

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Order Information

Cat. No.	Product Name	Description
LF08-  500	LumiFlash™ Ultima Chemiluminescent  Substrate, HRP	250mL soultion A +  250mL solution B

 

 

 

 

 

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Reference:

1. Kim, Yunho, et al. "Methylation-dependent regulation of HIF-1α stability restricts retinal and tumour angiogenesis." Nature communications 7 (2016): 10347.

2. Lim, Chae-Seok, et al. "PKCα-mediated phosphorylation of LSD1 is required for presynaptic plasticity and hippocampal learning and memory." Scientific reports 7.1 (2017): 1-15.

3. Lee, I-Ta, et al. "Protective mechanisms of resveratrol derivatives against TNF-α-induced inflammatory responses in rat mesangial cells." Cytokine 113 (2019): 380-392.

4. Cho, Min Young, et al. "Induction of DEAD Box helicase 5 in early adipogenesis is regulated by Ten-eleven translocation 2." Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids (2020): 158684.