TOPBlunt PCR Cloning Kit is suitable for cloning Pfu polymerase amplified PCR products with blunt ends at room temperature in 5min. The vector provided in this kit contains topoisomerase covalently coupled to its ends, which allows efficient cloning of blunt-ended DNA fragments to the vector without using a regular ligase.
Figure 1. Colony PCR results from colonies carrying different sized inserts.
PCR products of 300 bp (50 ng/µl) , 1 kb (50 ng/µl), 2 kb (100 ng/µl) and 3 kb (150 ng/µl) were ligated to TOPBlunt PCR Cloning Vectors, and colony PCR was performed using M13 primers.
For successful clones, the PCR result should be ~202 bp plus the insert size.
M : Gen100 DNA Ladder
TOPBlunt Vector Information
TOPBlunt PCR Cloning Kit
(Including competent cell)
TOPBlunt PCR Cloning Vector
(not including competent cell)
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