The GeneMark First stranded cDNA Synthesis Kit (SAMscript) is provided with all components required to perform first-strand cDNA synthesis. SAMscript Reverse Transcriptase (SAMscript RTase) is genetically engineered version of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RTase) which is a frequent choice for cDNA synthesis because of its ease of use and low intrinsic RNase H activity. In the cDNA synthesis step, RNA was reverse transcribed by SAMscript RTase to produce its cDNA up to 5 kb. The RNase Inhibitor supplied with the kit can protect RNA from degradation.
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