The GeneMark First stranded cDNA Synthesis Kit (SuperSAMscript) is provided with all components required to perform first-strand cDNA synthesis. SuperSAMscript Reverse Transcriptase (SuperSAMscript RTase) is genetically engineered version of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RTase) which lacks a 3’ to 5’ exonucleolytic proofreading function and has a weak RNase H activity as compared to AMV reverse transcriptase, and is useful for full-length cDNA amplification. In the cDNA synthesis step, RNA was reverse transcribed by SuperSAMscript RTase to produce its cDNA up to 20 kb from messenger RNA. The RNase Inhibitor supplied with the kit can protect RNA from degradation..
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