Transtep™ RNA Marker, This molecular weight marker is a unique ladder comprised of 10 transcription products, varying in size from 0.5 kb to 9.0 kb. The standards can be used for Northern Blots and are visualized by ethidium bromide staining. The storage buffer for this marker is 0.1mM EDTA solution. For evaluation by electrophoresis, 2-4 μg of the marker will generate 10 distinct bands on a 1% denaturing agarose gel with ethidium bromide staining. In preparation for electrophoresis, all of the markers should be mixed with an appropriate loading buffer (for denaturing gel electrophoresis), heated to 65°C for 10 minutes, and then placed on ice.
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