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Antibodies

Table of Contents

1. Overview of ELISA (enzyme-linked immunosorbent assay)
2. ELISA Formats
3. Antibodies and Other Probes for ELISA
   1. 3.1 Secondary Conjugates for ELISA
   2. 3.2 HRP and Poly-HRP Secondary Antibodies
   3. 3.3 HRP and AP Streptavidin Conjugates
4. ELISA Substrates and Detection Strategies
   1. 4.1 Colorimetric ELISA Substrates
   2. 4.2 Fluorimetric ELISA Substrates
   3. 4.3 Chemiluminescent ELISA substrates
5. Product Ordering Information

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Apoptosis & Proliferation

Apoptosis and Necrosis

Annexin-V Conjugates, Caspase Activity Assays, TUNEL Assays, GSH Assays

Annexin-V Staining

Phosphatidylserine (PS), Annexin-V & Conjugates, Flow Cytometry

Caspases

Caspase Substrates & Inhibitors, Caspase Binding Assays, Live Cell Caspase Acivity Assays

Cell Cycle and Proliferation

Live & Fixed Cell Cycle Assays, Cell Tracking & Proliferation Indicators

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Calcium Indicators

Fluo 8®, Cal-520®, Cal-630™, Rhod-4™, Ratiometric Indicators, Salt & Dextran Conjugates

Intracellular pH

Acidic pH Indicators, Near-Neutral pH Indicators, Vesicle pH Indicators (Dextran Conjugates)

Intracellular Ions

Calcium Indicators, Zinc Indicators, Anion Indicators, Ratiometric Indicators, FLIPR® Assays

Screen Quest™ Products

FLIPR® Assays, Intracellular Ion Assays, Ion Channels, cAMP Assays, Glucose & Fatty Acid Uptake Assays, MDR Assays

G-Protein-Coupled Receptors (GPCR)

Calcium Signaling, Drug Discovery, GPCR Transfected Cell Lines

NAD/NADH and NADP/NADPH

NAD(P) Assays, NAD(P)H Assays, NAD(P)/NAD(P)H Ratio Assays, Total NAD(P) and NAD(P)H Assays

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CD3

Fluorescent CD3 Conjugates, T Cell Biomarker, Flow Cytometry, IHC

CD4 (Leu3, T4)

Fluorescent CD4 Conjugates, T Helper Cell Biomarker, HIV

CD8 (TCR, Leu2, T8)

Fluorescent CD8 Conjugates, Cytotoxic T Cell Biomarker, Flow Cytometry, WB, IF

CD14

Fluorescent CD14 Conjugates, Macrophage & Monocyte Biomarker, Flow Cytometry

CD19 (Leu12, B-Lymphocyte Antigen B4)

Fluorescent CD19 Conjugates, B Cell & Follicular Denritic Cell Biomarker, Lymphoma

CD34 (Mucosialin)

Fluorescent CD34 Conjugates, Hematopoietic Progenitor Cell Biomarker, Angiosarcoma, Leukemia

CD40 (TNFRSF5)

Fluorescent CD40 Conjugates, B Cell Biomarker, Hyper-IgM Immunodeficiency

CD45 (Protein tyrosine phosphatase, PTPRC)

Fluorescent CD45 Conjugates, Hematopoietic Cell Biomarker, Flow Cytometry

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Cell Proliferation Assays

Cell Tracking & Proliferation Indicators

Cell Metabolism & Cell Signaling

Cell metabolism, Cell Signaling, Biomolecule Quantification, Enzyme Cofactor Quantification, Oxidative stress

Cell Structures and Organelles

Fluorescence Microscopy, Organelle Labeling Probes & Kits, Actin, ER, Golgi, Lysosomes, Mitochondria, Nucleus, Plasma Membrane

Cell Viability

Exclusion Assays, Reduction Assays, Fluorescent Esterase Substrates

Membrane Potential and Channels

Slow & Fast Membrane Potential Probes, FLIPR® Membrane Potential Assays

Mitochondrial Membrane Potential

JC-1 & JC-10™ Membrane Potential Probes, Flow Cytometry

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Alkaline Phosphatase (ALP)

AP Labeling Kits & Reagents, AP Conjugates & Substrates, AP ELISA Assay Kits

Dehydrogenases

Glucose-6-Phosphate Dehydrogenase Assays, Lactate Dehydrogenase Assays

Horseradish Peroxidase (HRP) and Poly-HRP

HRP Labeling Kits & Reagents, HRP Conjugates & Substrates, HRP ELISA Assay Kits

Kinases

Protein Kinase Assays, Enterokinase Assays, Pyrophosphate (PPi)

Luciferases

Firefly Luciferase, Guassia Luciferase, Renilla Luciferase, Reporter Gene Assays

Proteases

Universal Protease Activity Assay, Matrix Metalloproteinase Assays, Proteasome 20S Assays, Renin, Peptidases

Transferases

Alanine Aminotransferase, Aspartate Aminotransferase, HDAC Activity Assay

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Classic Dyes

Coumarins, Fluoresceins, Rhodamines, Cyanines, Indocyanine Greens

iFluor Dyes and Kits

Reactive Dyes, Antibody Labeling Kits, iFluor™ Secondaries, Annexin-V Conjugates, Phalloidin Conjugates

Indocyanine Green

ICG Reactive Dyes, In Vivo Molecular Imaging, Infrared Fluorescence

mFluor Dyes and Kits

Reactive Dyes, CD Antibody Conjugates, Annexin-V Conjugates, Flow Cytometry

PE and APC

PE & APC Labeling Kits & Reagents, Tandem Dyes, Streptavidin Conjugates

Tide Fluor Dyes and Kits

Oligo & Peptide Fluorescent Labeling Reagents, FRET Assays, Molecular Beacon Design

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Peptide and Oligonucleotide Labeling

Tide Fluor™ Dyes, Click Chemistry Dyes, Dye CPGs, Dye Phosphoramidites

Tide Fluor Dyes and Kits

Oligo & Peptide Fluorescent Labeling Reagents, FRET Assays, Molecular Beacon Design

Tide Quencher Dyes

Non-Fluorescent Quenchers, FRET Assays, Molecular Beacon Design

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Gram Staining

Gram Staining Kits & Reagents, Fixed Cell Stains, Live Cell Stains

MycoLight™ Dyes and Kits

Bacterial Viability Assays, Bacterial Membrane Potential Kits, Bacteria Imaging Assays

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DNA and RNA

Nucleic Acid Quantification & Detection Reagents, Gene Expression Analysis, dsDNA, RNA, PCR, Gel Stains

PCR Detection of Viral DNA/RNA

Reverse Transcriptase PCR, End-point PCR, Real-time PCR, Viral Diagnostics

Peptide and Oligonucleotide Labeling

Tide Fluor™ Dyes, Click Chemistry Dyes, Dye CPGs, Dye Phosphoramidites

Tide Fluor Dyes and Kits

Oligo & Peptide Fluorescent Labeling Reagents, FRET Assays, Molecular Beacon Design

Tide Quencher Dyes

Non-Fluorescent Quenchers, FRET Assays, Molecular Beacon Design

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Dax J2™ Sensors

Nitric Oxide Sensors, Ratiometric Sensors, Peroxynitrite Assays

OxiVision™ Sensors

Hydrogen Peroxide Sensors, Cell Imaging, Flow Cytometry

Reactive Oxygen Species (ROS)

Hydrogen Peroxide, Hydroxyl Radical, TBHP, Hypochlorous Acid, Superoxide Anion Detection

Glutathione (GSH and GSSG)

Evaluate Oxidative Stress, GSH Assays, GSH/GSSG Ratio Assays,

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