Ultracentrifuge-Independent Method Separate Four Subcellular Protein Fractions

FracPRO™ Protein Fractionation Kit is a product with highly specialized fractionation buffers which can target specific subcellular compartment and with ultracentrifuge-independent method to separate four subcellular protein fractions, including nuclear, cytosol, membrane/organelle, and cytoskeletal fractions in a detergent-based procedure. It can simultaneously preserve the structural integrity of the proteins and can directly be used in SDS-PAGE, enzyme analyses, gel mobility shift assays and other procedures.

Special Characteristic

• Compatible : Variable sample application including cultured cells and mammalian tissues
• Quick : Separate and collect four subcellular protein fractions in short time
• Simple : Isolate high quality protein fraction with easy procedure 

Product Detail :

Figure 1. The fractionation efficacy of Human leukemia cell line (K562) by FracPRO™ Protein Fractionation Kit.

The showed result detected by Western blot. (A) Heat shock protein 70 antibody (B) ATPase antibody (C) Histone deacetylase 2 antibody (D) Vimentin antibody. M: Pre-stained protein marker; Cy.: cytosol fraction; Mem.: membrane / organelle fraction; Nu.: nuclear fraction; Sk.: cytoskeletal fraction.

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