GTPO01 TOPfast PCR Cloning Kit

GTPO01

TOPfast PCR Cloning Kit

廠牌:GeneMark
供應商:賽恩斯生物科技股份有限公司
聯絡電話:02-28110076(代表號)
定價:與我們聯絡

快速詢價

3894

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TOPfast PCR Cloning Kit is suitable for cloning Taq polymerase amplified PCR products (with A-tail overhangs) at room temperature in 5 min. The vector provided in this kit contains topoisomerase covalently coupled to its ends, which allows efficient ligation of DNA fragments with A-tail overhangs to the vector without using a regular ligase.

產品資訊

《快速簡單》

  • TOPO cloning 系統
  • 不需使用Ligase反應,僅需5分鐘即可完成 ligation反應。
  • 使用Ampicillin、藍白篩選。《好用、高效率》
  • Insert 長度至少可達3 kb

《好用、高效率》

  • 載體帶有單一EcoR I切位, 容易轉殖 (subclone)。                  

Figure 1.  Colony PCR results from colonies carrying different sized inserts.

PCR products of 300 bp (50 ng/µl)、1 kb (50 ng/µl) 、2 kb (100 ng/µl) and 3 kb (150 ng/µl) were ligated to TOPfast PCR Cloning Vectors, and colony PCR was performed using M13 primers.

For successful clones, the PCR result should be ~202 bp plus the insert size.

M : Gen-KB DNA Ladder

TOPfast Vector Information

產品內容物

   
1 vial of 20 μl TOPfast Vector (10 ng/μl)
1 vial of 20 μl 6X TOPfastTM Buffer
1 vial of 5 μl Control insert DNA (1 kb)
21 vials of 100 μl DH5α Competent Cell
1 vials 5 μl Control Plasmid
1 vial of 400 μl M13 Control primer mix
1 vial of 7.5 ml SOC medium
1 vial of 1 ml Sterile water 
訂購資訊
     
Products: Cat. No. Size

TOPfast PCR Cloning Kit

(Including competent cell)

GTOP01-20

GTOP01-40

20 r

40 r

TOPfast PCR Cloning Vector

(not including competent cell)

GTOP01-V20

GTOP01-V40

20 r

40 r

 


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