First stranded cDNA Synthesis Kit (AMV)
The GeneMark First stranded cDNA Synthesis Kit (AMV) is provided with all components required to perform first-strand cDNA synthesis. Avian Myeloblastosis Virus Reverse Transcriptase (AMV RTase) catalyzes the polymerization of DNA using DNA, RNA or DNA:RNA hybrids as templates. Besides possessing 5’-3’ DNA polymerase activity, the enzyme also possesses partial RNase H activity, which breaks apart RNA:DNA hybrids. It is used primarily for the synthesis of first and second strand complementary DNA (cDNA) and primer extensions. In the cDNA synthesis step, RNA is reverse transcribed by AMV RTase to produce its cDNA up to 1.2 kb. The RNase Inhibitor supplied with the kit can protect RNA from degradation.
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