TOPfast PCR Cloning Kit is suitable for cloning Taq polymerase amplified PCR products (with A-tail overhangs) at room temperature in 5 min. The vector provided in this kit contains topoisomerase covalently coupled to its ends, which allows efficient ligation of DNA fragments with A-tail overhangs to the vector without using a regular ligase.

產品資訊

《快速簡單》

• TOPO cloning 系統
• 不需使用Ligase反應，僅需5分鐘即可完成 ligation反應。
• 使用Ampicillin、藍白篩選。《好用、高效率》
• Insert 長度至少可達3 kb

《好用、高效率》

• 載體帶有單一EcoR I切位, 容易轉殖 (subclone)。                  

Figure 1.  Colony PCR results from colonies carrying different sized inserts.

PCR products of 300 bp (50 ng/µl)、1 kb (50 ng/µl) 、2 kb (100 ng/µl) and 3 kb (150 ng/µl) were ligated to TOPfast PCR Cloning Vectors, and colony PCR was performed using M13 primers.

For successful clones, the PCR result should be ~202 bp plus the insert size.

M : Gen-KB DNA Ladder

TOPfast Vector Information

產品內容物

1 vial of 20 μl	TOPfast Vector (10 ng/μl)
1 vial of 20 μl	6X TOPfastTM Buffer
1 vial of 5 μl	Control insert DNA (1 kb)
21 vials of 100 μl	DH5α Competent Cell
1 vials 5 μl	Control Plasmid
1 vial of 400 μl	M13 Control primer mix
1 vial of 7.5 ml	SOC medium
1 vial of 1 ml	Sterile water

訂購資訊       Products: Cat. No. Size TOPfast PCR Cloning Kit (Including competent cell) GTOP01-20 GTOP01-40 20 r 40 r TOPfast PCR Cloning Vector (not including competent cell) GTOP01-V20 GTOP01-V40 20 r 40 r